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Flow Cytometry and Cell Sorting (Springer Lab Manuals)From Springer
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The analysis and sorting of large numbers of cells with a fluorescence-activated cell sorter (FACS) was first achieved some 30 years ago. Since then, this technology has been rapidly developed and is used today in many laboratories.
A Springer Lab Manual Review of the First Edition:
"This is a most useful volume which will be a welcome addition for personal use and also for laboratories in a wide range of disciplines. Highly recommended."
CYTOBIOS
- Sales Rank: #2426068 in Books
- Published on: 2010-02-19
- Original language: English
- Number of items: 1
- Dimensions: 10.00" h x .86" w x 7.00" l, 1.88 pounds
- Binding: Paperback
- 355 pages
From the Back Cover
Flow cytometric analysis of molecular, biochemical, genetic and developmental parameters using cellular fluorescence techniques as well as fluorescence-activated (FACS) or magnetic (MACS) cell sorting technologies provide unique options for molecular and cellular biology. In recent years, these technologies have been considerably advanced.
In this second edition, all chapters have been updated according to the recent improvements and modifications. Further, new protocols have been added, such as on magnetic selection, magnetofluorescent liposomes, the cytometry of secreted products and microbead assays, as well as reporter gene assays for cytometry and cell selection. The recent technical developments allow diagnostic differentiation of cells according to specific gene expression, identification of rare disease-associated cells and isolation of well-defined cells at high purity for cell therapies. In basic research, analysis and isolation of cells according to the correlated expression of up to 10 gene products, expressed within, on or around the cell have become possible.
From the reviews of the 1st edition:
"This is a most useful volume which will be a welcome addition for personal use and also for laboratories in a wide range of disciplines. Highly recommended and well worth purchasing." (Cytobios, GB)
"Full practical details and useful hints are given. This book will prove to be very useful in the lab." (Comp Biochem Physiol A, GB)
"..the book provides a good overview of many of the uses of flow cytometry and would certainly be a good starting point for any of the topics covered. It is easy to read and as such would provide a good introduction to newcomers to flow cytometry." (Austr J Med Science)
Most helpful customer reviews
9 of 9 people found the following review helpful.
A book for the beginner and a review for the experienced
By A Customer
This is the first book I purchased for flow cytometry after checking out 3 unsatisfactory books on the same subject from the library. I am a clinician by training and so I needed a book that explains the basic background of the method, PLUS detailed procedures to follow, and explanation on how to alter those procedures to suit one's experiment. This book is written and presented in a way that makes it easy to understand even if one has no prior trianing in flow-cytometry. It does not have so much technical jargon that a newbie would not be able to understand. Everything is clear and well-explained. I have "debugged" some of my current procedures using this book. Chapter 3 (Immunofluroescence: Basic Considerations) is a must-read: the validity of one's results seem to largely depend on factors discussed in this chapter. Included also is a list of internet resouce for cytometry, although some of websites have been outdated. Overall a very nice reference, but as in all lab procedures-related books, one book never covers everything you need to know, so I use this book by Radbruch in conjuction with others.
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